Method variation in lactate dehydrogenase isoenzyme determination.
نویسنده
چکیده
Differences between methods for determining lactate dehydrogenase isoenzymes are illustrated, which can account for discrepant results. They should be taken into consideration in the interpretation of test results for the diagnosis of myocardial infarction.
منابع مشابه
Automated kinetic determination of lactate dehydrogenase isoenzymes in serum.
A steady-state kinetic method has been revised for measuring lactate dehydrogenase isoenzyme activities, which relates the inhibition of heart-type isoenzyme activity to the overall isoenzyme composition of the enzyme subunits. The method depends on the pH-dependent formation of an inhibitory ternary complex by the heart-type isoenzyme with NAD+ and pyruvate (if the reaction is measured by NADH...
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We have evaluated the determination of lactate dehydrogenase (EC 1.1.1.27) isoenzyme 1 activity by chemical inhibition of the other isoenzymes with perchlorate and with 1,6-hexanediol. In the hexanediol method, we studied the effect of the duration of incubation with the inhibitor; a 5-min incubation yielded results closest to those of an immunochemical technique (Isomune-LD). The perchlorate m...
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Five lactate dehydrogenase (LD; L-lactate:NAD oxidoreductase, EC 1.1.127) isoenzymes are demonstrated by electrophoresis in normal human serum. Altered activities of these isoenzymes have been associated with pathological conditions, so that their determination has become a useful diagnostic aid. Abnormal LD-isoenzyme patterns with one and two extra isoenzyme bands have been reported in serum s...
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The creatine kinase and lactate dehydrogenase isoenzyme pattern were determined in the serum of normal and untreated rats, rabbits, dogs, monkeys and pigs. The relative distribution of all isoenzymes in the serum and an electrophoretic pattern for each animal species are presented. The isoenzyme serum pattern showed a great variation between the species. The diagnostic value of serum creatine k...
متن کاملInfluence of hemolysis on the serum lactate dehydrogenase-1/lactate dehydrogenase-2 ratio as determined by an accurate thin-layer agarose electrophoresis procedure.
The accuracy of our lactate dehydrogenase (EC 1.1.1.27) isoenzyme procedure, based on electrophoresis in thin-layer agarose, has previously been assessed (Clin. Chem. 22: 1995, 1976). We now show that the method is similarly accurate and precise for the specific measurements of lactate dehydrogenase isoenzyme-1 and -2. We have also determined the lactate dehydrogenase isoenzyme pattern in perip...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 27 4 شماره
صفحات -
تاریخ انتشار 1981